What kind of chromatography cold cabinet should be chosen for protein purification system?
Release Date:
2025-05-20 09:53
What kind of chromatography refrigerator should be chosen for a protein purification system?
First, we need to understand 2 basic issues.
1. Regarding temperature: When conducting related experiments with a protein purification system, biological samples can easily deteriorate if the temperature is above 10 °C. How should this be handled? Additionally, when the temperature is below 0 °C, samples and reagents are prone to freezing, and chromatography columns may be damaged. How should the temperature be controlled then? To address these issues, the internal cabinet temperature range is generally set to 2-8 °C.
2. Regarding humidity: Inside the chromatography cabinet, humidity may increase during experiments due to the evaporation of water from some reagents; when humidity is very high (humidity greater than 90%RH), the equipment's control circuit board is prone to moisture regain, which could lead to short-circuiting issues in the protein purification system's control circuit. How should we deal with this?
According to national standards for environmental conditions for electrical and electronic products, the requirements for temperature and humidity are: reference warehouse temperature and relative humidity for electronic components must meet the following requirements: Temperature: -5-30 °C, Relative humidity 20%-75%RH. In practical operation, a range of 40-65%RH is generally considered most suitable. We can refer to this humidity control standard to protect the protein purification system and extend the service life of the protein purification system equipment.
Considering the above two aspects, the best choice for equipping a protein purification system with a chromatography refrigerator is one that can control both temperature and humidity (e.g., Xiamen Guoyi Premium Chromatography Refrigerator). This not only meets experimental needs but also protects high-value equipment.
Secondly, in addition to the two basic requirements above, we also need to consider multiple aspects during actual procurement, such as temperature control stability, cooling efficiency, safety, durability, and usability.
Temperature control stability: Consider the temperature control performance of the chromatography cabinet from angles such as temperature uniformity, stability, and accuracy; generally, the smaller the temperature fluctuation inside the cabinet, the better, as it is more favorable for experimental data.
Cooling efficiency: Can be referenced from cooling method and dehumidification time.
Durability: Can be referenced from materials, components, and control system performance. For example, selecting Secop, the best industry brand for compressor components, using 304 stainless steel for the cabinet material, and considering foaming thickness, etc.
Safety: Can be referenced from the alarm system, such as high/low humidity alarms and temperature fluctuations.
Usability: Can be considered from aspects such as automatic defogging, automatic defrosting, safety door lock, door frame heating, UV lamp, lighting, chromatography column, test data ports, number of power outlets and waterproofing, and shelf flexibility.
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